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Electrochemistry shows great potential for biosensing due to its easy-operation (typically with a three-electrode system) and rapid measuring, particularly for portable substrates so they can be integrated as electrode conveniently with the system. The electrical and physical properties make metallic nanoparticles suitable materials for electrochemical biosensors. Metallic nanoparticles can not only facilitate electron transfer with high conductivity but also immobilize molecules of interest. Among metals, silver is the best conductor and silver nanoparticles (AgNPs) can be easily obtained through chemical synthesis. Conductive carbon tapes are a good candidate for deposition of metallic nanoparticles. They can not only make the substrates flexible and portable but also provide good conductivity during electrochemical measurements. They can be self-assembled with metallic nanoparticles. Therefore, in this work, double-sided adhesive carbon tapes coated with AgNPs are fabricated in a simple way. With the prepared conductive substrate as working electrode in a three-electrode system, urea and other proteins at low concentration were measured with cyclic voltammetry (CV). The results showed that carbon tapes coated with AgNPs were able to detect urea with strong signals and that different proteins on the substrates can also be identified. Moreover, urea can be distinguished from proteins or other samples such as plasma including several components on this substrate via EC biosensing.
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