Surface-enhanced Raman spectroscopy and localized surface plasmon resonance detection of hydrogen peroxide using plasmonic gels

Yun-Chu Chen; Kuan-Ying Chen; Kuo-Hsiu Hsu; Yih-Fan Chen

doi:10.1117/12.2509744

7 March 2019 Surface-enhanced Raman spectroscopy and localized surface plasmon resonance detection of hydrogen peroxide using plasmonic gels

Yun-Chu Chen, Kuan-Ying Chen, Kuo-Hsiu Hsu, Yih-Fan Chen

Author Affiliations +

Proceedings Volume 10894, Plasmonics in Biology and Medicine XVI; 108940R (2019) https://doi.org/10.1117/12.2509744
Event: SPIE BiOS, 2019, San Francisco, California, United States

Abstract

We will present surface enhanced Raman spectroscopy (SERS) and localized surface plasmon resonance (LSPR) detection of hydrogen peroxide (H₂O₂) using plasmonic gels. It has been known that reactive oxygen species (ROS), such as hydrogen peroxide, are involved in various biological processes, including metabolism, cell signaling, protein folding, biosynthesis, and host defense. Therefore, developing a simple and sensitive method for monitoring ROS levels is very important for live cell studies. Nevertheless, a challenge of utilizing SERS-based or LSPR-based method for molecular detection in complex fluids, such as cell culture media, is that a variety of molecules in sample solutions could interfere with detection results. In addition, when using SERS-based methods, the chance of having the target molecules at the SERS hot spots is reduced when other molecules are present in the solution. To enable detection of H₂O₂ in cell culture media, we have developed SERS and LSPR detection methods based on gels containing plasmonic nanoparticles. Since gels are filter-like materials, H₂O₂ can penetrate through the gels, but cells and large molecules such as proteins are blocked. We have successfully utilized these two methods to detect H₂O₂ in cell culture media without any sample pretreatment.

Citation Download Citation

Yun-Chu Chen, Kuan-Ying Chen, Kuo-Hsiu Hsu, and Yih-Fan Chen "Surface-enhanced Raman spectroscopy and localized surface plasmon resonance detection of hydrogen peroxide using plasmonic gels", Proc. SPIE 10894, Plasmonics in Biology and Medicine XVI, 108940R (7 March 2019); https://doi.org/10.1117/12.2509744

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available