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7 March 2019 Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers
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Abstract
The nano-assembly of charged polyelectrolytes via layer-by-layer (LbL) technology on porous silicon (PSi) interferometers is here demonstrated as an effective biofunctionalization approach for high-sensitivity/selectivity labelfree optical biosensing, using streptavidin/biotin affinity detection as case study. Nanostructured PSi interferometers are biofunctionalized with a nano-assembly of a positively-charged polyelectrolyte, namely, PAH (poly(allylamine hydrochloride)), and a negatively-charged biotinylated polyelectrolyte, namely, b-PMAA (poly(methacrylic acid)), via LbL technology. The nano-assembly is stable under operating conditions and enables the selective and sensitive detection of streptavidin with a sub-picomolar detection limit (namely, DL=0.6 pM), which is 105-fold lower than that achieved with PSi interferometers biofunctionalized using standard silane chemistry. Remarkably, the analytical performance achieved for LbL-biofunctionalized PSi interferometers is comparable to those of state-of-the-art label-free photonic and plasmonic platforms.
Conference Presentation
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
S. Mariani, V. Robbiano, L. M. Strambini, A. Debrassi, G. Egri, L. Dähne, and G. Barillaro "Layer-by-layer nano-assembly of charged polyelectrolytes for label-free optical biosensing with nanostructured materials: the case of nanostructured porous silicon interferometers", Proc. SPIE 10895, Frontiers in Biological Detection: From Nanosensors to Systems XI, 1089503 (7 March 2019); https://doi.org/10.1117/12.2508847
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