Different LED light treatments (monochromatic royal blue - RB, blue - B, green G, yellow - Y, red - R, deep red - DR, and polychromatic cold white - CW, white - W, warm white - WW, full spectrum - FS, and the newly constructed Sun box - SB) on potato plantlets in vitro were studied. Longwave monochromatic light sources cannot be used for micropropagation of virus-free material due to producing the fragile stems and poorly developed leaves. Two LED lights (WW and FS), were excellent sources for micropropagation. . Investigation of different light brightness in SB revealed the optimum at 135 μmol/с*m2 , which activated plantlet growth, and induced green and root mass accumulation. Such illumination parameters can be usefull for shortening the time of vegetation, rapid stolonization and increase of virusfree minitubers productivity.