Nano-biosensors based on dynamic light scattering

Alexander D. Levin; Maxim P. Nikitin; Mikhail K. Alenichev; Ekaterina B. Drozhzhennikova; Vitaly G. Grigorenko; Alina S. Ringaci; Irina P. Andreeva

doi:10.1117/12.2526055

21 June 2019 Nano-biosensors based on dynamic light scattering

Alexander D. Levin, Maxim P. Nikitin, Mikhail K. Alenichev, Ekaterina B. Drozhzhennikova, Vitaly G. Grigorenko, Alina S. Ringaci, Irina P. Andreeva

Author Affiliations +

Proceedings Volume 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV; 110600Y (2019) https://doi.org/10.1117/12.2526055
Event: SPIE Optical Metrology, 2019, Munich, Germany

Abstract

The two new approaches to dynamic light scattering (DLS) biosensing are presented. The first method based on the effect of the slowing-down of nanoparticles rotational diffusion due aggregation and the high sensitivity of the autocorrelation function (ACF) of depolarized component of the scattered light to the rotational diffusion coefficient. This way it is possible to significantly increase the sensitivity of the analysis. The second approach implements a competitive immunoassay in the DLS version, while increasing the analyte concentration reduces the degree of aggregation of the conjugates therefore leads to a decrease in the average hydrodynamic diameter. On the basis of the proposed approaches, nanosensors for the PSA tumor marker and the antibiotic cloramphenicol were developed.

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Alexander D. Levin, Maxim P. Nikitin, Mikhail K. Alenichev, Ekaterina B. Drozhzhennikova, Vitaly G. Grigorenko, Alina S. Ringaci, and Irina P. Andreeva "Nano-biosensors based on dynamic light scattering", Proc. SPIE 11060, Optical Methods for Inspection, Characterization, and Imaging of Biomaterials IV, 110600Y (21 June 2019); https://doi.org/10.1117/12.2526055

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