Light sheet microscopy of blood vessels in mouse brain in vivo

Ivan V. Fedosov; Oxana V. Semyachkina-Glushkovskaya; Valery V. Tuchin

doi:10.1117/12.2523883

3 June 2019 Light sheet microscopy of blood vessels in mouse brain in vivo

Ivan V. Fedosov, Oxana V. Semyachkina-Glushkovskaya, Valery V. Tuchin

Proceedings Volume 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions; 1106711 (2019) https://doi.org/10.1117/12.2523883
Event: International Symposium on Optics and Biophotonics VI: Saratov Fall Meeting 2018, 2018, Saratov, Russian Federation

Abstract

A method for intravital light sheet microscopy of blood vessels of mouse brain cortex is proposed. The use of tilted microscope lens mount and long working distance of microscope lens ensure high contrast images in both scattered light and fluorescence modes without immersion. Fluctuations of laser speckles related with the blood flow has been observed in scattered mode. In fluorescence mode distribution of Evans Blue dye over blood vessel cross-section was visualized.

Citation Download Citation

Ivan V. Fedosov, Oxana V. Semyachkina-Glushkovskaya, and Valery V. Tuchin "Light sheet microscopy of blood vessels in mouse brain in vivo", Proc. SPIE 11067, Saratov Fall Meeting 2018: Computations and Data Analysis: from Nanoscale Tools to Brain Functions, 1106711 (3 June 2019); https://doi.org/10.1117/12.2523883

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