Combination light-based therapies to treat pancreatic cancer: a proof of concept

A. Ney; I. Mahamed; A. Garcia-Sampedro; P. K. Selbo; P. Sancho; A. J. MacRobert; S. P. Pereira; P. Acedo

doi:10.1117/12.2525305

7 August 2019 Combination light-based therapies to treat pancreatic cancer: a proof of concept

A. Ney, I. Mahamed, A. Garcia-Sampedro, P. K. Selbo, P. Sancho, A. J. MacRobert, S. P. Pereira, P. Acedo

Proceedings Volume 11070, 17th International Photodynamic Association World Congress; 1107045 (2019) https://doi.org/10.1117/12.2525305
Event: 17th International Photodynamic Association World Congress, 2019, Cambridge, Massachusetts, United States

Abstract

Pancreatic ductal adenocarcinoma remains one of the worst types of cancers mainly due to its late diagnosis, lack of effective therapies for advance disease and high chemoresistance. Novel therapeutic options that could improve patient quality of life and overall survival are therefore imperative. In this study, we describe the use of an original strategy based on photochemical internalisation (PCI) technology for pancreatic cancer treatment. Subcellular localisation of the photosensitiser meso-tetraphenylporphine-disulfonate (TPPS2a) was performed in PANC-1 cells, showing its preferential accumulation in lysosomes. Treatments with increasing concentrations of the ribosome-inactivating protein saporin or TPPS2a alone were compared with PCI-saporin. Metabolic activity and cell viability of PANC-1 cells were determined 96h post-illumination by MTT and trypan blue assays, respectively. Our results show that PCI using the photosensitiser TPPS2a, synergistically enhances the cytotoxic effects of saporin in PANC- 1 cells and could offer more effective treatment options for pancreatic cancer.

Conference Presentation

Citation Download Citation

A. Ney, I. Mahamed, A. Garcia-Sampedro, P. K. Selbo, P. Sancho, A. J. MacRobert, S. P. Pereira, and P. Acedo "Combination light-based therapies to treat pancreatic cancer: a proof of concept", Proc. SPIE 11070, 17th International Photodynamic Association World Congress, 1107045 (7 August 2019); https://doi.org/10.1117/12.2525305

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