3D large-volume histological imaging of ex vivo tissue samples using inverted selective plane illumination microscopy (iSPIM)

Bihe Hu; Guang Li; J. Quincy Brown

doi:10.1117/12.2526875

19 July 2019 3D large-volume histological imaging of ex vivo tissue samples using inverted selective plane illumination microscopy (iSPIM)

Bihe Hu, Guang Li, J. Quincy Brown

Author Affiliations +

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110730K (2019) https://doi.org/10.1117/12.2526875
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

The use of iSPIM and optical-clearing enables 3D high-content histological imaging of large tissues with subcellular resolution and almost 2 mm imaging depth, providing 3D structural information for generating tissue models or for validating low-resolution imaging modalities.

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Bihe Hu, Guang Li, and J. Quincy Brown "3D large-volume histological imaging of ex vivo tissue samples using inverted selective plane illumination microscopy (iSPIM) ", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110730K (19 July 2019); https://doi.org/10.1117/12.2526875

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