Longitudinal monitoring of in-vivo mice mammary tumor progression using intravital fluorescence tomography and optical coherence tomography

Mehmet S. Ozturk; Ling Wang; Lucas M. Chaible; Marta G. Montero; Martin Jechlinger; Robert Prevedel

doi:10.1117/12.2527035

19 July 2019 Longitudinal monitoring of in-vivo mice mammary tumor progression using intravital fluorescence tomography and optical coherence tomography

Mehmet S. Ozturk, Ling Wang, Lucas M. Chaible, Marta G. Montero, Martin Jechlinger, Robert Prevedel

Author Affiliations +

Proceedings Volume 11073, Clinical and Preclinical Optical Diagnostics II; 110731B (2019) https://doi.org/10.1117/12.2527035
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Cancer prognosis and treatment efficacy are assessed by evaluating the hallmarks of a tumor which are volume change of the tumor and the vascular network reformation around a tumor. Non-invasive quantitative assessment of those indications, in-vivo, is still a challenge for traditional imaging modalities, owing to a large interrogation area and deep-seated molecular (fluorescence) signal in highly scattering, anatomically complex skin tissue. Currently available techniques either utilize surgically implanted imaging windows or conduct terminal experiments for each time point. The former is prone to inflammation at the implantation site thus interfering with the tumor microenvironment. The latter one is prone to sample variability thus results in a pseudo-longitudinal outcome for tumor development. Here, we combine Intravital Fluorescence Tomography (IFT) and Spectral Domain Optical Coherence Tomography (SD-OCT) for tumor imaging with a Non-invasive Intravital Imaging Window (NIIW) for tissue stabilization. This platform enabled us to follow tumor development non-invasively covering tumor initiation, development and regression on the same animal over months-long period. IFT-OCT multimodal imaging not only reveals tumor volume change but also skin anatomical features, and it is capable of revealing neo-vascularization around the tumor site. This platform thus serves as a useful non-invasive tool to explore future research questions pertaining to cancer biology in common fluorescence-based mouse models, such as tumor progression or treatment efficacy. In addition, our multi-modal platform alleviates the burden put on animals while imaging and reduce the experimental cost significantly.

Citation Download Citation

Mehmet S. Ozturk, Ling Wang, Lucas M. Chaible, Marta G. Montero, Martin Jechlinger, and Robert Prevedel "Longitudinal monitoring of in-vivo mice mammary tumor progression using intravital fluorescence tomography and optical coherence tomography", Proc. SPIE 11073, Clinical and Preclinical Optical Diagnostics II, 110731B (19 July 2019); https://doi.org/10.1117/12.2527035

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tumors

Skin

Optical coherence tomography

Fluorescence tomography

In vivo imaging

Multimodal imaging

Cancer

Show All Keywords

Keywords/Phrases

Search In:

Publication Years