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The detection of molecules by surface-enhanced Raman spectroscopy (SERS) is dependent on the nanomaterial used to induce the enhancement effect. This depends on a variety of parameters of the substrate such as the metal used for their creation, their shape, size and size distribution, concentration, as well as the parameters of the solution, such as packing of the nanoparticles, the complexity of the sample, the solvent, etc. It is most crucial, that the parameters are kept constant to provide uniformity of the enhancement. this is crucial for the development of SERS as a reliable and quantitative technique for bioanalysis. Here, we have developed the silver-core and gold-shell nanoparticles, to serve as the enhancement material. The fabrication phase involved constant concentrations of chemicals stability of the solution physical parameters like stirring and heating, and differed only in the perturbation of the reagents addition kinetics. These nanoparticles were investigated further with their ability to measure the solutions of 2-naphtalenethiol in DMSO, as model for testing the variability of the signal due to the enhancement and the kinetics of the nanoparticle-sample solution during a routine Raman measurement procedure. The results indicate vast difference in the preference of the 2-naphthalenethiol to come into contact with the nanoparticles and the partial enhancement of DMSO in most cases, with an almost complete by-pass of the solvent and direct detection of the 2-naphthalenethiol in one case. Moreover, the kinetics of the measurement solution, or its stability during measurement, is provided.
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