Myeloarchitecture of the murine neocortex visualized by intrinsic third-harmonic generation

Michael Redlich; Hyungsik Lim

doi:10.1117/12.2527064

22 July 2019 Myeloarchitecture of the murine neocortex visualized by intrinsic third-harmonic generation

Michael Redlich, Hyungsik Lim

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110760A (2019) https://doi.org/10.1117/12.2527064
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Myelination in the brain has been typically associated with the white matter. Recently, however, cortical myelination has gained much interest not only as a possible lesion of multiple sclerosis (MS) but also for its significance in the plasticity of the central nervous system (CNS). It is urgent to understand the functional role of cortical myelination and its involvement in the MS pathogenesis. Here we demonstrate a novel nonlinear optical contrast, namely third-harmonic generation (THG), as a basis of label-free imaging of cortical myelin in living animals. Using the fixed brain slices of fluorescently labeled transgenic mice, we examined the distribution of myelin in the normal brain. We measured the spatial organization of cortical myelin. Finally, the capability of THG microscopy was tested for visualizing cortical myelin in living, unlabeled animals.

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Michael Redlich and Hyungsik Lim "Myeloarchitecture of the murine neocortex visualized by intrinsic third-harmonic generation", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110760A (22 July 2019); https://doi.org/10.1117/12.2527064

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KEYWORDS

Brain

Visualization

Microscopy

Third-harmonic generation

Cerebral cortex

Neuroimaging

Green fluorescent protein

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