Fast P-THG microscopy for the characterization of biomaterials

Joséphine Morizet; Guillaume Ducourthial; Willy Supatto; Arthur Boutillon; Renaud Legouis; Marie-Claire Schanne-Klein; Chiara Stringari; Emmanuel Beaurepaire

doi:10.1117/12.2527209

22 July 2019 Fast P-THG microscopy for the characterization of biomaterials

Joséphine Morizet, Guillaume Ducourthial, Willy Supatto, Arthur Boutillon, Renaud Legouis, Marie-Claire Schanne-Klein, Chiara Stringari, Emmanuel Beaurepaire

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 1107613 (2019) https://doi.org/10.1117/12.2527209
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

The application range of P-THG microscopy has been so far restricted to studies on molecular order and anisotropy of static specimen removed from their biological environment. Slow polarization commutation limits the investigation of highly dynamic systems because of motion artifacts. Here we have developed a new fast-P-THG microscope enabling efficient in vivo studies in dynamic biological samples. Our P-THG scheme benefits from a built-in EOM that switch polarization states at kilohertz between image lines to provide artefact-free P-THG images with micrometric resolution. Furthermore, we have developed a fast Fourier analysis enabling rapid P-THG processing to quantify lipid order and angular maps. We demonstrated that fast-P-THG is suitable in two major applications. Using first a linear polarization configuration, fast P-THG imaging revealed molecular order changes in MLVs undergoing phase-transition upon heating despite sample distortions. Anisotropy properties of small endogenous microparticles swimming in the otolith cavity embryos were also reported in early zebrafish embryos. A second configuration with linear-circular polarization commutation enabled efficient detection of birefringent media such as anisotropic vesicles in C-elegans gut cells.

Citation Download Citation

Joséphine Morizet, Guillaume Ducourthial, Willy Supatto, Arthur Boutillon, Renaud Legouis, Marie-Claire Schanne-Klein, Chiara Stringari, and Emmanuel Beaurepaire "Fast P-THG microscopy for the characterization of biomaterials", Proc. SPIE 11076, Advances in Microscopic Imaging II, 1107613 (22 July 2019); https://doi.org/10.1117/12.2527209

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
3 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Polarization

Microscopy

Anisotropy

Biological research

In vivo imaging

Microscopes

Multiphoton microscopy

Show All Keywords

Keywords/Phrases

Search In:

Publication Years