Probing chemosensitivity and energy metabolism in patients-derived colorectal cancer cells

Irina Druzhkova; Maria Lukina; Nadezhda Ignatova; Varvara Dudenkova; Marina Shirmanova; Vladimir Zagainov; Nikolay Kiselev; Lyubov' Shimolina; Elena Zagaynova

doi:10.1117/12.2527021

22 July 2019 Probing chemosensitivity and energy metabolism in patients-derived colorectal cancer cells

Irina Druzhkova, Maria Lukina, Nadezhda Ignatova, Varvara Dudenkova, Marina Shirmanova, Vladimir Zagainov, Nikolay Kiselev, Lyubov' Shimolina, Elena Zagaynova

Author Affiliations +

Proceedings Volume 11076, Advances in Microscopic Imaging II; 110761K (2019) https://doi.org/10.1117/12.2527021
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

Colorectal cancer is one of the most commonly diagnosed and poorly responding to chemotherapy types of cancer, which emphasizes the importance of personalized approach to treatment selection. Short-term primary cell cultures established from patients’ tumors represent a valuable model for testing drug response. In this study, we developed protocols for generation of the short-term primary cell cultures from colorectal cancer tissue and assessment their chemosensitivity using MTT test. Additionally, we showed the possibility of metabolic analysis of patient-derived cancer cells using fluorescence lifetime imaging (FLIM) of autofluorescent cofactor NAD(P)H. Since FLIM of NAD(P)H demonstrates the potential to detect early responses to cancer treatment, we assume that this method, alone or in combination with MTT assay, can be used for choosing the optimal chemotherapy for patients.

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Irina Druzhkova, Maria Lukina, Nadezhda Ignatova, Varvara Dudenkova, Marina Shirmanova, Vladimir Zagainov, Nikolay Kiselev, Lyubov' Shimolina, and Elena Zagaynova "Probing chemosensitivity and energy metabolism in patients-derived colorectal cancer cells ", Proc. SPIE 11076, Advances in Microscopic Imaging II, 110761K (22 July 2019); https://doi.org/10.1117/12.2527021

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