Durable 3D test standards from mouse retina for performance testing of high-resolution optical coherence tomography systems

Álvaro Barroso; Peter Heiduschka; Gerburg Nettels-Hackert; Steffi Ketelhut; Björn Kemper; Jürgen Schnekenburger

doi:10.1117/12.2527163

19 July 2019 Durable 3D test standards from mouse retina for performance testing of high-resolution optical coherence tomography systems

Álvaro Barroso, Peter Heiduschka, Gerburg Nettels-Hackert, Steffi Ketelhut, Björn Kemper, Jürgen Schnekenburger

Author Affiliations +

Proceedings Volume 11078, Optical Coherence Imaging Techniques and Imaging in Scattering Media III; 110781V (2019) https://doi.org/10.1117/12.2527163
Event: European Conferences on Biomedical Optics, 2019, Munich, Germany

Abstract

For performance testing of high-resolution label-free optical coherence tomography (OCT) systems, durable test standards with optical properties similar to native biological tissues are highly desirable. Therefore, we explored the establishment of ex-vivo 3D samples from dissected mouse retina. To achieve high stability, dissected tissue parts were embedded in EPON resin. Results from comparative investigations with OCT on murine retina in agarose demonstrate that by utilization of appropriate EPON embedding protocols durable samples with adequate refractive index properties can be created in which even tiny structures near the OCT resolution limits can be detected.

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Álvaro Barroso, Peter Heiduschka, Gerburg Nettels-Hackert, Steffi Ketelhut, Björn Kemper, and Jürgen Schnekenburger "Durable 3D test standards from mouse retina for performance testing of high-resolution optical coherence tomography systems", Proc. SPIE 11078, Optical Coherence Imaging Techniques and Imaging in Scattering Media III, 110781V (19 July 2019); https://doi.org/10.1117/12.2527163

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