Optical trapping and manipulation with a low NA objective through 3D printed micro-mirrors (Conference Presentation)

Gheorghe Cojoc; Andrea Bertoncini; Paul Müller; Jochen Guck; Carlo Liberale

doi:10.1117/12.2529532

9 September 2019 Optical trapping and manipulation with a low NA objective through 3D printed micro-mirrors (Conference Presentation)

Gheorghe Cojoc, Andrea Bertoncini, Paul Müller, Jochen Guck, Carlo Liberale

Author Affiliations +

Proceedings Volume 11083, Optical Trapping and Optical Micromanipulation XVI; 110830C (2019) https://doi.org/10.1117/12.2529532
Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States

Abstract

In the last three decades, optical trapping techniques were heavily employed for contactless trapping and manipulation of biological samples. Dual-beam laser traps (DBLT) proved their convenience and became widely used as biophysical tool once a simplified experimental setup was proposed. This simplification was achieved by replacing the two objectives with optical fibers to deliver the two counter-propagating laser beams. However, fiber alignment can be inconvenient, time consuming and requires a lot of practice. Here, we present a novel way to overcome these issues by combining reconfigurable diffractive optical elements (DOE) and two photon lithography (2PL), using a single low NA objective. A single laser beam is divided into several beams by displaying a DOE on a spatial light modulator (SLM). This allows us to dynamically reconfigure the number of the beams, their shape, and relative 3D alignment. Furthermore, we use 3D printed micro-mirrors to direct the laser beams against each other and obtain a DBLT. The micro-mirrors were fabricated on top of a coverslip, by means of 2PL. Our preliminary results show the ability to trap dielectric and biological samples and their full 3D manipulation in a DBLT configuration. The ability to use DOEs to set the number of beams and their shape allow this technique to be coupled with novel forms of microscopy.

Conference Presentation

Citation Download Citation

Gheorghe Cojoc, Andrea Bertoncini, Paul Müller, Jochen Guck, and Carlo Liberale "Optical trapping and manipulation with a low NA objective through 3D printed micro-mirrors (Conference Presentation)", Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 110830C (9 September 2019); https://doi.org/10.1117/12.2529532

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Micromirrors

Objectives

Optical tweezers

Diffractive optical elements

Beam shaping

Spatial light modulators

Dielectrics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years