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9 September 2019 FORMA: a high-performance algorithm for the calibration of optical tweezers (Conference Presentation)
Giovanni Volpe, Laura Pérez García, Alejandro V. Arzola, Jaime Donlucas Perez, Giorgio Volpe
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Proceedings Volume 11083, Optical Trapping and Optical Micromanipulation XVI; 110831U (2019) https://doi.org/10.1117/12.2530626
Event: SPIE Nanoscience + Engineering, 2019, San Diego, California, United States
Abstract
The accurate measurement of microscopic force fields is crucial in many branches of science and technology, from biophotonics and mechanobiology to microscopy and optomechanics. These forces are often probed by analysing their influence on the motion of Brownian particles. Here we introduce a powerful algorithm for microscopic force reconstruction via maximum-likelihood-estimator analysis (FORMA) to retrieve the force field acting on a Brownian particle from the analysis of its displacements [1]. FORMA estimates accurately the conservative and non-conservative components of the force field with important advantages over established techniques, being parameter-free, requiring ten-fold less data and executing orders-of-magnitude faster. We demonstrate FORMA performance using optical tweezers, showing how, outperforming other available techniques, it can identify and characterise stable and unstable equilibrium points in generic force fields. Thanks to its high performance, FORMA can accelerate the development of microscopic and nanoscopic force transducers for physics, biology and engineering. [1] García, Laura Pérez, Jaime Donlucas Pérez, Giorgio Volpe, Alejandro V. Arzola, and Giovanni Volpe. "High-performance reconstruction of microscopic force fields from Brownian trajectories." Nature Communications 9, no. 1 (2018): 5166. https://doi.org/10.1038/s41467-018-07437-x
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Giovanni Volpe, Laura Pérez García, Alejandro V. Arzola, Jaime Donlucas Perez, and Giorgio Volpe "FORMA: a high-performance algorithm for the calibration of optical tweezers (Conference Presentation)", Proc. SPIE 11083, Optical Trapping and Optical Micromanipulation XVI, 110831U (9 September 2019); https://doi.org/10.1117/12.2530626
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KEYWORDS
Optical tweezers
Calibration
Optical calibration
Particles
Reconstruction algorithms
Biomedical optics
Microscopy
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