Phase-contrast x-ray tomography of neuronal tissue at laboratory sources with sub-micron resolution

Marina Eckermann; Mareike Töpperwien; Anna-Lena Robisch; Franziska van der Meer; Christine Stadelmann; Tim Salditt

doi:10.1117/12.2529098

9 September 2019 Phase-contrast x-ray tomography of neuronal tissue at laboratory sources with sub-micron resolution

Marina Eckermann, Mareike Töpperwien, Anna-Lena Robisch, Franziska van der Meer, Christine Stadelmann, Tim Salditt

Proceedings Volume 11112, X-Ray Nanoimaging: Instruments and Methods IV; 111120R (2019) https://doi.org/10.1117/12.2529098
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

Recently, progress has been achieved in implementing phase contrast tomography of soft biological tissues at laboratory sources.^1-4 This opens up novel opportunities for three-dimensional (3d) histology based on x-ray computed tomography (μ- and nanoCT) in direct vicinity of hospitals and biomedical research institutions. Combining novel x-ray generation and detection techniques with suitable phase reconstruction algorithms, 3d histology can be obtained even of unstained tissue of the central nervous system, as shown for example for biopsies and autopsies of human cerebellum.^{5, 6} Depending on the setups, in particular source, detector, and geometric parameters, laboratory-based tomography can be implemented at very different sizes and length scales. In the present work, we investigate to which extent 3d histology of neuronal tissue can take advantage of cone-beam geometry at high magnification M using a nanofocus x-ray source (Excillum AB) with a minimum spot size of 300 nm, in combination with a single-photon counting camera. Tightly approaching the source spot with the biopsy punch, we achieve high M of ≈ 10¹-10², high flux density and can exploit the superior efficiency of this detector technology. Results are compared with those obtained at a microfocus rotating-anode x-ray tomography setup equipped with a high resolution detector, i.e. an low-M geometry.

Citation Download Citation

Marina Eckermann, Mareike Töpperwien, Anna-Lena Robisch, Franziska van der Meer, Christine Stadelmann, and Tim Salditt "Phase-contrast x-ray tomography of neuronal tissue at laboratory sources with sub-micron resolution", Proc. SPIE 11112, X-Ray Nanoimaging: Instruments and Methods IV, 111120R (9 September 2019); https://doi.org/10.1117/12.2529098

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KEYWORDS

Tomography

Tissues

Sensors

Signal to noise ratio

X-rays

Phase contrast

Image quality

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