Biological laboratory x-ray microscopy

Mikael Kördel; Emelie Fogelqvist; Valentina Carannante; Björn Önfelt; Hemanth K. N. Reddy; Martin Svenda; Jonas A. Sellberg; Komang G. Y. Arsana; Ulrich Vogt; Hans M. Hertz

doi:10.1117/12.2531165

9 September 2019 Biological laboratory x-ray microscopy

Mikael Kördel, Emelie Fogelqvist, Valentina Carannante, Björn Önfelt, Hemanth K. N. Reddy, Martin Svenda, Jonas A. Sellberg, Komang G. Y. Arsana, Ulrich Vogt, Hans M. Hertz

Author Affiliations +

Proceedings Volume 11112, X-Ray Nanoimaging: Instruments and Methods IV; 111120T (2019) https://doi.org/10.1117/12.2531165
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

Zone-plate-based soft x-ray microscopes operating in the water window allow high-resolution and high-contrast imaging of intact cells in their near-native state. Laboratory-source-based x-ray microscopes are an important complement to the accelerator-based instruments, providing high accessibility and allowing close integration with other cell-biological techniques. Here we present recent biological applications using the Stockholm laboratory water-window x-ray microscope, which is based on a liquid-nitrogen-jet laser-plasma source. Technical improvements to the microscope in the last few years have resulted in increased x-ray flux at the sample and significantly improved stability and reliability. In addition to this, vibrations in key components have been measured, analyzed and reduced to improve the resolution to 25 nm half-period. The biological applications include monitoring the development of carbon-dense vesicles in starving human embryonic kidney cells (HEK293T), imaging the interaction between natural killer (NK) cells and HEK293T target cells, and most recently studying a newly discovered giant DNA virus and the process of viral replication inside a host amoeba. All biological imaging was done on cryo-frozen hydrated samples in 2D and in some cases 3D.

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Mikael Kördel, Emelie Fogelqvist, Valentina Carannante, Björn Önfelt, Hemanth K. N. Reddy, Martin Svenda, Jonas A. Sellberg, Komang G. Y. Arsana, Ulrich Vogt, and Hans M. Hertz "Biological laboratory x-ray microscopy", Proc. SPIE 11112, X-Ray Nanoimaging: Instruments and Methods IV, 111120T (9 September 2019); https://doi.org/10.1117/12.2531165

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