High-resolution IR-based imaging with third-order sum-frequency generation microscopy (Conference Presentation)

Eric O. Potma; Adam M. Hanninen; Richard C. Prince

doi:10.1117/12.2530523

9 September 2019 High-resolution IR-based imaging with third-order sum-frequency generation microscopy (Conference Presentation)

Eric O. Potma, Adam M. Hanninen, Richard C. Prince

Author Affiliations +

Proceedings Volume 11122, Ultrafast Nonlinear Imaging and Spectroscopy VII; 111220I (2019) https://doi.org/10.1117/12.2530523
Event: SPIE Optical Engineering + Applications, 2019, San Diego, California, United States

Abstract

We studied the use of vibrationally resonant, third-order sum-frequency generation (TSFG) for imaging of biological samples. We found that laser-scanning TSFG provides vibrationally sensitive imaging capabilities of lipid droplets and structures in sectioned tissue samples. Although the contrast is based on the infrared-activity of molecular modes, TSFG images exhibit a high lateral resolution of 0.5 μm or better. We observed that the imaging properties of TSFG resemble the imaging properties of coherent anti-Stokes Raman scattering (CARS) microscopy, offering a nonlinear infrared alternative to coherent Raman methods. TSFG microscopy holds promise as a high-resolution imaging technique in the fingerprint region where coherent Raman techniques often provide insufficient sensitivity.

Conference Presentation

Citation Download Citation

Eric O. Potma, Adam M. Hanninen, and Richard C. Prince "High-resolution IR-based imaging with third-order sum-frequency generation microscopy (Conference Presentation)", Proc. SPIE 11122, Ultrafast Nonlinear Imaging and Spectroscopy VII, 111220I (9 September 2019); https://doi.org/10.1117/12.2530523

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