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17 May 2019 Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit
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Proceedings Volume 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019); 1117043 (2019) https://doi.org/10.1117/12.2534145
Event: Fourteenth National Conference on Laser Technology and Optoelectronics, 2019, Shanghai, China
Abstract
The detection of senescent cells becomes increasing important for tumor therapy and drug screening. Here a light sheet microfluidic cytometer with a disposable hydrodynamic focusing unit is developed for two dimensional (2D) light scattering measurements of single cells. The mixed polystyrene microspheres of 3.87 and 2.0 μm in diameter are successfully differentiated by our 2D light scattering microfluidic cytometer. The application of the 2D light scattering microfluidic cytometry for the label-free analysis of senescent cells without any labeling or staining is demonstrated by measurements of H2O2-treated U87 cells. Our light sheet-based 2D light scattering microfluidic cytometer is easy to assemble with a disposable hydrodynamic unit, which may find wild applications in clinics for label-free cell classification.
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Meiai Lin, Shuyu Zhang, Qiao Liu, Changshun Shao, and Xuantao Su "Label-free analysis of senescent cells by light sheet microfluidic cytometry with a disposable hydrodynamic focusing unit", Proc. SPIE 11170, 14th National Conference on Laser Technology and Optoelectronics (LTO 2019), 1117043 (17 May 2019); https://doi.org/10.1117/12.2534145
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