Three-dimensional macro-scale micro-structure imaging with deep ultraviolet excitation

Jiaming Guo; Camille Artur; Jason L. Eriksen; David Mayerich

doi:10.1117/12.2538364

19 November 2019 Three-dimensional macro-scale micro-structure imaging with deep ultraviolet excitation

Jiaming Guo, Camille Artur, Jason L. Eriksen, David Mayerich

Proceedings Volume 11186, Advanced Optical Imaging Technologies II; 111860O (2019) https://doi.org/10.1117/12.2538364
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Deep ultraviolet light excites many common fluorophores and is heavily absorbed by biological samples, making it ideal for facial histology of fresh biological samples. Microscopy with ultraviolet surface excitation (MUSE) provides high-resolution diagnostic images comparable to slide-based imaging. However, current implementations of MUSE are limited to the facial surface of the sample, restricting the availability of three-dimensional tissue structure. Our work extends MUSE imaging by providing inexpensive three dimensional images at resolutions comparable to confocal microscopy with the throughput of wide-field imaging. This approach allows comprehensive imaging of macro-scale samples by eliminating constraints on sample size and imaging depth. In this paper, we discuss recent advances in milling with ultraviolet excitation, with a focus on complex neurological structures such as cellular and microvascular networks.

Citation Download Citation

Jiaming Guo, Camille Artur, Jason L. Eriksen, and David Mayerich "Three-dimensional macro-scale micro-structure imaging with deep ultraviolet excitation", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111860O (19 November 2019); https://doi.org/10.1117/12.2538364

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KEYWORDS

Ultraviolet radiation

Tissues

Microscopy

3D image processing

Deep ultraviolet

Confocal microscopy

Image segmentation

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