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19 November 2019 Nonlinear focal modulation microscopy based on interleaved reconstruction
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Abstract
Nonlinear focal modulation microscopy (NFOMM) is a really simple and effective super-resolution imaging method based on phase modulation with high-intensity illumination, which can extend the effective spatial-frequency bandwidth of the imaging system and achieve a transverse resolution of ~ 60 nm (~λ/10). However, multiple images under different illumination pattern is needed, which result in limited imaging speed. A novel interleaved reconstruction method is proposed to increase the frame rate without any change in the raw data of NFOMM. Since allowing easy integration with confocal microscopes, we anticipate this method will be a useful observation tool in the biological community and other research field.
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Yuchen Chen, Chengfeng Zhang, and Cuifang Kuang "Nonlinear focal modulation microscopy based on interleaved reconstruction", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 1118611 (19 November 2019); https://doi.org/10.1117/12.2537324
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