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19 November 2019 Quantitative phase microscopy based on color-multiplexed single-shot Fourier ptychography
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Abstract
We present a single-shot quantitative phase imaging (QPI) method based on color-multiplexed Fourier ptychographic microscopy (FPM). Three light-emitting diode (LED) elements with respective R/G/B channels in a programmable LED array illuminate the specimen simultaneously, providing triangle oblique illuminations matching the numerical aperture (NA) of the objective lens precisely. A single color image sensor records the light transmitted through the specimen, and three monochromatic intensity images at each color channel are then separated and utilized to recover the phase of the specimen. After one-step deconvolution based on the phase contrast transfer function, the obtained initial phase map is further refined by the FPM-based iterative recovery algorithm to overcome pixel-aliasing and improve the phase recovery accuracy. The high-speed, high- throughput QPI capabilities of the proposed approach are demonstrated by achieving a half-pitch resolution of 345 nm across a wide FOV of 1.33 mm2 at camera-limited frame rates (50 fps).
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Jiasong Sun, Chao Zuo, and Qian Chen "Quantitative phase microscopy based on color-multiplexed single-shot Fourier ptychography", Proc. SPIE 11186, Advanced Optical Imaging Technologies II, 111861K (19 November 2019); https://doi.org/10.1117/12.2554998
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