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20 November 2019 Label-free imaging of lymph nodes with stimulated Raman scattering microscopy
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Abstract
Lymph nodes are an important secondary lymphoid organ in the lymphatic system. It is the major site of B cells, T cells and other white blood cells, leading to the function of identifying and fighting infection. To observe the structure of the lymph node, it is necessary to label it with dyes or fluorescent probes. Multi-color labeling are required to label various cell types and structures, which may be difficult under conditions such as in vivo labeling. Stimulated Raman scattering microscopy (SRS) is a burgeoning label-free biological imaging technique that has been used in many areas, such as tumor detection, pharmacokinetics, and neuroscience. Here, we developed a method of label-free imaging of various cell types and structures in lymph nodes with SRS. Collagenous fibers in the lymph node capsule, subcapsular sinus macrophages, B cells, blood vessels and white blood cells were observed in fresh lymph nodes. Based on the visualization of lymph node structure, we prospected its applications in cancer research, aging research and extracellular matrix research.
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yuanzhen Suo, Wenlong Yang, Fake Lu, and Xiaoliang Sunney Xie "Label-free imaging of lymph nodes with stimulated Raman scattering microscopy", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111900J (20 November 2019); https://doi.org/10.1117/12.2536264
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