Ultrahigh-resolution stimulation by femtosecond laser reveals existence and regulation mechanism of nuclear Ca2+ store

Xiaoying Tian; Hao He

doi:10.1117/12.2537703

20 November 2019 Ultrahigh-resolution stimulation by femtosecond laser reveals existence and regulation mechanism of nuclear Ca²⁺ store

Xiaoying Tian, Hao He

Author Affiliations +

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111901N (2019) https://doi.org/10.1117/12.2537703
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Nuclear Ca²⁺ regulates many signaling pathways including NFAT, CREB, MEF that are essential to cell immunity, development and differentiation. In current stage, there is no specified biochemical drugs to regulate nuclear Ca²⁺ individually. The existence of nuclear Ca²⁺ store remains unknown. In this study, we show the femtosecond-laser stimulation can precisely regulate nuclear and other subcellular Ca²⁺ stores exclusively. The stimulated Ca²⁺ store can be released but other subcellular Ca²⁺ stores are not interfered by femtosecond laser. By using this method, we present the evidence of the endogenous Ca²⁺ store inside nucleus. The nuclear Ca²⁺ store volume and release mechanism are clarified. By photostimulation, nuclear Ca²⁺ store can be released and impact the calcium in endoplasmic reticulum (ER), suggesting a Ca²⁺ transmission channel between nucleus and ER. The permeability of nuclear pore complex (NPC) to Ca²⁺ at different Ca²⁺ levels is also found different. This study indicates the existence of nuclear Ca²⁺ store and the final mapping of subcellular Ca²⁺ stores. This result thus is of great significance to the cell calcium theory, Ca²⁺ signaling, Ca²⁺-related cell processes, and further other Ca²⁺-related fields.

Citation Download Citation

Xiaoying Tian and Hao He "Ultrahigh-resolution stimulation by femtosecond laser reveals existence and regulation mechanism of nuclear Ca²⁺ store", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111901N (20 November 2019); https://doi.org/10.1117/12.2537703

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