Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing

Shuyu Zhang; Meiai Lin; Xuantao Su

doi:10.1117/12.2537425

20 November 2019 Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing

Shuyu Zhang, Meiai Lin, Xuantao Su

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111902A (2019) https://doi.org/10.1117/12.2537425
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

Microfluidic cytometry has led to great progress in the field of single cell analysis. Here a light scattering microfluidic cytometer with dual-channel three-dimensional (3D) hydrodynamic focusing is developed, which potentially enables single cell or particle measurements with high-throughput. The light sheet illumination is introduced to provide a uniform excitation. Two dimensional (2D) light scattering patterns of label-free single cells or particles are imaged by a CMOS sensor via an objective lens (10x). The focusing effect of our dual-channel 3D hydrodynamic focusing unit is visualized. Simultaneous measurements of standard beads with different concentrations and sizes are performed. Our results demonstrate that the light scattering microfluidic cytometer with dual-channel 3D hydrodynamic focusing is promising for parallel, label free analysis of various cells with an enhanced throughput.

Citation Download Citation

Shuyu Zhang, Meiai Lin, and Xuantao Su "Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902A (20 November 2019); https://doi.org/10.1117/12.2537425

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