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20 November 2019 Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing
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Abstract
Microfluidic cytometry has led to great progress in the field of single cell analysis. Here a light scattering microfluidic cytometer with dual-channel three-dimensional (3D) hydrodynamic focusing is developed, which potentially enables single cell or particle measurements with high-throughput. The light sheet illumination is introduced to provide a uniform excitation. Two dimensional (2D) light scattering patterns of label-free single cells or particles are imaged by a CMOS sensor via an objective lens (10x). The focusing effect of our dual-channel 3D hydrodynamic focusing unit is visualized. Simultaneous measurements of standard beads with different concentrations and sizes are performed. Our results demonstrate that the light scattering microfluidic cytometer with dual-channel 3D hydrodynamic focusing is promising for parallel, label free analysis of various cells with an enhanced throughput.
© (2019) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Shuyu Zhang, Meiai Lin, and Xuantao Su "Label-free light scattering microfluidic cytometry using dual-channel 3D hydrodynamic focusing", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902A (20 November 2019); https://doi.org/10.1117/12.2537425
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