Non-invasive 3D real time observation of physiological traits during the embryonic development of insects

Hao Tan; Ya Su; Liya Wei; X. Steve Yao; Tongtong Mai; Xu Li

doi:10.1117/12.2537479

20 November 2019 Non-invasive 3D real time observation of physiological traits during the embryonic development of insects

Hao Tan, Ya Su, Liya Wei, X. Steve Yao, Tongtong Mai, Xu Li

Author Affiliations +

Proceedings Volume 11190, Optics in Health Care and Biomedical Optics IX; 111902F (2019) https://doi.org/10.1117/12.2537479
Event: SPIE/COS Photonics Asia, 2019, Hangzhou, China

Abstract

The visualization of embryonic development is important for the study of basic physiological development. It is also indispensable for evaluating the effects of modification and editing of genetic engineering. Compared with traditional invasive methods of monitoring embryo development, optical coherence tomography (OCT) has its great advantages, such as high resolution, real time and non-invasive. These characteristics of OCT make it possible to observe a living embryo in its whole growth development. In this paper, we use OCT to monitor the embryonic development of locusts noninvasively and reconstruct the two-dimensional and three-dimensional image of the embryo at different development stages. The phenomena of anatrepsis, revolution and katatrepsis are observed. The in-vitro observations of the prior studies with invasive methods are confirmed by the in-vivo experimental results. We hope OCT is a useful tool for the understanding of the embryonic development of insects.

Citation Download Citation

Hao Tan, Ya Su, Liya Wei, X. Steve Yao, Tongtong Mai, and Xu Li "Non-invasive 3D real time observation of physiological traits during the embryonic development of insects", Proc. SPIE 11190, Optics in Health Care and Biomedical Optics IX, 111902F (20 November 2019); https://doi.org/10.1117/12.2537479

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available