Electrospun diamond-silk membranes for biosensing applications

Asma Khalid; Amanda N. Abraham; Dongbi Bai; Amit Jadhav; Denver Linklater; Duy Nguyen; Alex D. Matusica; Chaitali Dekiwadia; Achini K. Vidanapathirana; Philipp Reineck; Christina A. Bursill; Elena Ivanova; Shadi Houshyar; Andrew Greentree; Brant Gibson

doi:10.1117/12.2539276

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30 December 2019 Electrospun diamond-silk membranes for biosensing applications

Asma Khalid, Amanda N. Abraham, Dongbi Bai, Amit Jadhav, Denver Linklater, Duy Nguyen, Alex D. Matusica, Chaitali Dekiwadia, Achini K. Vidanapathirana, Philipp Reineck, Christina A. Bursill, Elena Ivanova, Shadi Houshyar, Andrew Greentree, Brant Gibson

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Proceedings Volume 11202, Biophotonics Australasia 2019; 1120211 (2019) https://doi.org/10.1117/12.2539276
Event: ANZCOP, 2019, Melbourne, Australia

Abstract

This work reports nanodiamond-silk membranes as an optical platform for biosensing and cell growth applications. The hybrid structure was fabricated through electrospinning and mimics a 2D scaffold with high porosity. The negatively charged nitrogen vacancy (NV-) centres in diamond exhibits optically detected magnetic resonance (ODMR), which enables sensing of temperature variations. The NV- centre, as reported in literature, provides a shift of 74 kHz in the ODMR frequency per degree rise in temperature. For our hybrid membranes, we have however observed that the embedded NV- centre provide a greater shift of 95±5 kHz/K in the ODMR frequency. This higher shift in the frequency will result in improved temperature sensitivity enabling the tracking of thermal variations in the biologically relevant window of 25-50 ºC. The thermal conductivity of silk and diamond-silk hybrid will be explored to investigate this enhanced temperature sensing ability of diamond. The hybrid diamond-silk membranes are found to be hydrophilic with a contact angle of (65±2)º. The biocompatibility of the membranes is tested both in vitro in skin keratinocyte (HaCaT) cells and in vivo in a live mouse wound model. The membranes did not induce any toxicity to the cell growth and survival. Moreover, we observed resistance towards the growth and attachment of bacteria.

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Asma Khalid, Amanda N. Abraham, Dongbi Bai, Amit Jadhav, Denver Linklater, Duy Nguyen, Alex D. Matusica, Chaitali Dekiwadia, Achini K. Vidanapathirana, Philipp Reineck, Christina A. Bursill, Elena Ivanova, Shadi Houshyar, Andrew Greentree, and Brant Gibson "Electrospun diamond-silk membranes for biosensing applications", Proc. SPIE 11202, Biophotonics Australasia 2019, 1120211 (30 December 2019); https://doi.org/10.1117/12.2539276

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