Software package for off-axis digital holographic microscopy imaging processing

Tienan Xu; Xuefei He; Zhiduo Zhang; Samantha Montague; Elizabeth Gardiner; Woie Ming Lee

doi:10.1117/12.2539541

30 December 2019 Software package for off-axis digital holographic microscopy imaging processing

Tienan Xu, Xuefei He, Zhiduo Zhang, Samantha Montague, Elizabeth Gardiner, Woie Ming Lee

Proceedings Volume 11202, Biophotonics Australasia 2019; 112021C (2019) https://doi.org/10.1117/12.2539541
Event: ANZCOP, 2019, Melbourne, Australia

Abstract

Digital Holographic Microscopy (DHM) has emerged as a powerful imaging tool due to its ability to provide quantitative sample morphological data in a label-free manner. Off-axis holography further opens possibilities of numerical approaches to image analysis and data processing. However, this involves computationally heavy workflows that limit the tool’s usability in biomedical research. In this paper, a software package is developed for off-axis hologram reconstruction and processing. The software can perform a variety of bioimaging operations including imaging, region of interest selection, automated reconstruction, and data extraction, with a user-friendly graphical user interface. Originally programmed in MATLAB, the software can be installed to use in common lab-based Microsoft Windows computers. The software can be easily adopted to work with customized off-axis DHM setups, aiming to increase the efficiency of DHM’s bioimaging workflow in the community. To demonstrate this software, blood platelet experiments were conducted to show quantitative volume change of thrombus formation.

Citation Download Citation

Tienan Xu, Xuefei He, Zhiduo Zhang, Samantha Montague, Elizabeth Gardiner, and Woie Ming Lee "Software package for off-axis digital holographic microscopy imaging processing", Proc. SPIE 11202, Biophotonics Australasia 2019, 112021C (30 December 2019); https://doi.org/10.1117/12.2539541

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available