Spatial frequency domain imaging of hemodynamic parameters in a murine model of hindlimb ischemia

Katherine Leyba; Sandhya Vasudevan; Thomas D. O'Sullivan; Craig J. Goergen

doi:10.1117/12.2541944

25 February 2020 Spatial frequency domain imaging of hemodynamic parameters in a murine model of hindlimb ischemia

Katherine Leyba, Sandhya Vasudevan, Thomas D. O'Sullivan, Craig J. Goergen

Proceedings Volume 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020; 112150C (2020) https://doi.org/10.1117/12.2541944
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Near infrared spectroscopy (NIRS) is used for a wide variety of applications due to its noninvasiveness, ease of use, quick acquisition time, and affordability. In this study we investigate the effectiveness of spatial frequency domain imaging (SFDI), a widefield noncontact quantitative NIRS technique, to assess peripheral artery disease progression in vivo. SFDI measures absolute (i.e. optical scattering-corrected) tissue concentrations of oxy- hemoglobin and deoxyhemoglobin, and therefore total hemoglobin concentration and tissue oxygen saturation. Female apolipoprotein E-deficient mice (n=9) underwent femoral artery ligation to induce unilateral ischemia in the left hindlimb. Brie y, the left femoral artery was exposed and ligated via suture midway between the aortic trifurcation and division into the saphenous and popliteal arteries. SFDI was acquired for both the ischemic and control limbs over 4 weeks to track changes in vascular perfusion. We also acquired high frequency pulsed-wave Doppler ultrasound images and performed histological analysis, both of which confirmed occlusion of the left femoral artery post-ligation. The ischemic to control ratio for tissue oxygen saturation was 0.96±0.06 at baseline and 0.86±0.10 at day 1, 0.94±0.06 at day 3, 0.95±0.14 at day 7, 0.91±0.09 at day 14, 0.90±0.09 at day 21, and 1.01±0.09 at day 28. These results demonstrate the ability of NIRS to detect a decrease in tissue oxygen saturation in the ischemic limb within a week of ischemic injury, followed by recovery at 4 weeks post-ligation. Our work provides evidence for the potential of SFDI to accurately and noninvasively quantify peripheral artery disease progression in a preclinical murine model.

Conference Presentation

Citation Download Citation

Katherine Leyba, Sandhya Vasudevan, Thomas D. O'Sullivan, and Craig J. Goergen "Spatial frequency domain imaging of hemodynamic parameters in a murine model of hindlimb ischemia", Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150C (25 February 2020); https://doi.org/10.1117/12.2541944

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available