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9 March 2020 Analysis of coronary microvasculature and myocardial nuclei orientation in embryonic hearts (Conference Presentation)
Maryse Lapierre-Landry, Hana Kolesova, Yehe Liu, Michiko Watanabe, Michael W. Jenkins
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Proceedings Volume 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020; 112150T (2020) https://doi.org/10.1117/12.2544442
Event: SPIE BiOS, 2020, San Francisco, California, United States
Abstract
New techniques are needed to study the coronary microvasculature in the embryo and to quantify the differences between healthy and diseased coronary development. We combine our custom optical clearing method (LIMPID), fluorescent staining with DiI and DAPI, and 3D confocal microscopy to visualize the coronary vasculature at two developmental stages (day 9, 13) in quails. We discovered a highly organized coronary vessel network that is aligned with surrounding myocardium cells even at early developmental stages. By characterizing the normal heart vasculature, this experiment provides a baseline for future studies on how diseases affect coronary and myocardial orientation in the embryo.
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Maryse Lapierre-Landry, Hana Kolesova, Yehe Liu, Michiko Watanabe, and Michael W. Jenkins "Analysis of coronary microvasculature and myocardial nuclei orientation in embryonic hearts (Conference Presentation)", Proc. SPIE 11215, Diagnostic and Therapeutic Applications of Light in Cardiology 2020, 112150T (9 March 2020); https://doi.org/10.1117/12.2544442
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KEYWORDS
Heart
Confocal microscopy
3D image processing
Fourier transforms
Image segmentation
Optical clearing
Quantitative analysis
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