All-optical correlative micro-spectroscopies in the investigation of stromal collagen morpho-mechanics

Raffaella Mercatelli; Sara Mattana; Laura Capozzoli; Fulvio Ratto; Francesca Rossi; Roberto Pini; Daniele Fioretto; Francesco S. Pavone; Silvia Caponi; Riccardo Cicchi

doi:10.1117/12.2543742

19 February 2020 All-optical correlative micro-spectroscopies in the investigation of stromal collagen morpho-mechanics

Raffaella Mercatelli, Sara Mattana, Laura Capozzoli, Fulvio Ratto, Francesca Rossi, Roberto Pini, Daniele Fioretto, Francesco S. Pavone, Silvia Caponi, Riccardo Cicchi

Author Affiliations +

Proceedings Volume 11218, Ophthalmic Technologies XXX; 112180U (2020) https://doi.org/10.1117/12.2543742
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Tissue morpho-mechanics is gaining increasing in various fields, because it targets the relationship between morphological features and mechanical properties in biological tissues, which plays an important role in various fields including biology, medicine, pathology, tissue engineering, and regenerative medicine. The intimate connection between morphological, biochemical and mechanical properties in biological tissues requires a multimodal correlative approach for their exhaustive investigation. In this study, we used Second-Harmonic Generation in combination with Brillouin and Raman micro-spectroscopy in order to correlate collagen morphology at the ultrastructural level with its biomechanical and biochemical features. In particular, by imaging human corneal tissue samples with our multimodal approach, we demonstrated that the peculiar mechanical properties of corneal lamellae in the anterior portion of the corneal stroma are due to a different supramolecular organization, rather than to a different biochemical composition. This result opens new insights in the study and interpretation of corneal biomechanics thanks to the possibility of non-invasively correlating lamellar morphology with visco-elastic properties. The proposed method opens the way to the non-invasive assessment of corneal morpho-mechanics and holds the potential to be used for both diagnostic and follow-up purposes in pathologies that affect corneal biomechanics.

Citation Download Citation

Raffaella Mercatelli, Sara Mattana, Laura Capozzoli, Fulvio Ratto, Francesca Rossi, Roberto Pini, Daniele Fioretto, Francesco S. Pavone, Silvia Caponi, and Riccardo Cicchi "All-optical correlative micro-spectroscopies in the investigation of stromal collagen morpho-mechanics", Proc. SPIE 11218, Ophthalmic Technologies XXX, 112180U (19 February 2020); https://doi.org/10.1117/12.2543742

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Second-harmonic generation

Collagen

Raman spectroscopy

Cornea

Tissues

Microscopy

Tissue optics

Show All Keywords

Keywords/Phrases

Search In:

Publication Years