Combined scattering and fluorescent measurement of the retina in vivo reveals disease-dependent changes in intrinsic fluorophores of retinal pigment epithelium (Conference Presentation)

Ratheesh Kumar Meleppat; Gabriel Peinado; Kaitryn E. Ronning; Sarah J. Karlen; Pengfei Zhang; Edward N. Pugh Jr.; Robert J. Zawadzki

doi:10.1117/12.2550163

9 March 2020 Combined scattering and fluorescent measurement of the retina in vivo reveals disease-dependent changes in intrinsic fluorophores of retinal pigment epithelium (Conference Presentation)

Ratheesh Kumar Meleppat, Gabriel Peinado, Kaitryn E. Ronning, Sarah J. Karlen, Pengfei Zhang, Edward N. Pugh Jr., Robert J. Zawadzki

Author Affiliations +

Proceedings Volume 11218, Ophthalmic Technologies XXX; 112181D (2020) https://doi.org/10.1117/12.2550163
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

A combined scattering and autofluorescence (AF) measurement from the retinal pigment epithelium (RPE) was investigated to study the changes in the AF granules in an Abca4-/- mouse (murine model of Stargardt disease). The directional scattering was measured with an optical coherence tomography (OCT), whereas the multi-color fundus AF spectra was measured with a spectrometer-integrated scanning laser ophthalmoscope. Increased scattering from the RPE in Abca4-/- mouse relative to control was well correlated with the elevated AF spectra and indicated the changes in RPE fluorophores. Ex vivo studies based on scanning confocal and electron microscopy were performed to validate the in vivo findings.

Conference Presentation

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Ratheesh Kumar Meleppat, Gabriel Peinado, Kaitryn E. Ronning, Sarah J. Karlen, Pengfei Zhang, Edward N. Pugh Jr., and Robert J. Zawadzki "Combined scattering and fluorescent measurement of the retina in vivo reveals disease-dependent changes in intrinsic fluorophores of retinal pigment epithelium (Conference Presentation)", Proc. SPIE 11218, Ophthalmic Technologies XXX, 112181D (9 March 2020); https://doi.org/10.1117/12.2550163

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KEYWORDS

Scattering

In vivo imaging

Scatter measurement

Retina

Luminescence

Ophthalmology

Multimodal imaging

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