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19 February 2020 A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models
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Abstract
A paired-agent fluorescent molecular imaging strategy is presented as a method to measure drug target engagement in whole tumor imaging. The protocol involves dynamic imaging of a pair of targeted and control imaging agents prior to and following antibody therapy. Simulations demonstrated that antibody “drug target engagement” can be estimated within a 15%-error over a wide range of tumor physiology (blood flow, vascular permeability, target density) and antibody characteristics (affinity, binding rates). Experimental results demonstrated the first in vivo detection of binding site barrier, highlighting the potential for this methodology to provide novel insights in drug distribution/binding imaging.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Elif Kayaalp-Nalbant, Cody Rounds, Negar Sadeghipour, Boyu Meng, Margaret R. Folaron, Chandrika Haldar, Rendall R. Strawbridge, Kimberley S. Samkoe, Scott C. Davis, and Kenneth M. Tichauer "A paired-agent fluorescent molecular imaging strategy for quantifying antibody drug target engagement in in vivo window chamber xenograft models", Proc. SPIE 11219, Visualizing and Quantifying Drug Distribution in Tissue IV, 112190G (19 February 2020); https://doi.org/10.1117/12.2545182
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