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19 February 2020 Combined fluorescence and optoacoustic imaging for monitoring the efficiency of photodynamic therapy with BPD-based nanoconstructs
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Abstract
A novel approach to monitor photosensitizer accumulation and photobleaching in the course of photodynamic therapy (PDT) with the use of nanoconstructs based on the simultaneous fluorescence (FL) and optoacoustic (OA) imaging is implemented. A liposome nanoconstructs employed in this studies contain benzoporphyrin derivatives (BPD) which serve as a photosensitizer and secondly, as a fluorophore, and the fluorescent IRDye800 dye acting as an additional contrasting agent due to its high quantum yield. FL provides visualization of BPD and IRDye800 distribution, while OA principle allows for BPD-absorption based imaging of tumor and its vascular environment. We demonstrate the results of a preliminary in vivo study with combined FL and OA custom-made setups on a NUDE mouse with human glioblastoma U- 87. The results of this studies show a hemorrhage in the tumor area on the OA images obtained @532 nm after PDT that is not visually detected, but confirmed with the following histological verification. Fast nanoconstructs accumulation (< 10 min) was observed using FL imaging with the concentration in tumor only 10% higher than in surrounding tissues. We believe that the ratio of nanoconstructs accumulation in tumor can be significantly increased using target approach.
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I. V. Turchin, M. Kirillin, D. Kurakina, A. Orlova, V. Plekhanov, E. Sergeeva, P. Subochev, E. Sergeev, V. Perekatova, A. Nerush, D. Yuzhakova, E. Kiseleva, M. Shirmanova, S. Bano, S. Mallidi, and T. Hasan "Combined fluorescence and optoacoustic imaging for monitoring the efficiency of photodynamic therapy with BPD-based nanoconstructs", Proc. SPIE 11220, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XXIX, 112200V (19 February 2020); https://doi.org/10.1117/12.2565087
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