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11 March 2020 Nanoconcentrations of 1,9-dimethylmethylene blue (DMMB) associated to laser, LED or polarized light are highly effective on AmPDT carried out in aerobes and aerotolerant anaerobes Gram-positive bacteria
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Abstract
Antimicrobial Photodynamic Therapy (AmPDT) is an alternative to conventional treatments of local infections such as the use of antibiotics, which may lead to the development of resistance. AmPDT in addition of requiring the use of a photosensitizer it also needs a light source executed. In search of an efficient and cheap procedure, we aimed to assess the efficacy of using 1,9-dimethylmethylene blue (DMMB) as photosensitizer associated with the use of either Laser (λ660 nm), LED (λ632 ± 2 nm) or a multispectral polarized light (λ400 – 2000 nm) using different energy densities to kill E. faecalis (Laser or LED) or S. aureus (PL) in vitro. For this, 300 (S. aureus) or 3.32 ηg/ml (E. faecalis) of DMBB and energy densities of 5 (PL) or 18 J/cm2 (Laser/LED) were used. Five minutes of pre-incubation time was used. CFU count (S. aureus) or the most probable number method (MPN) (E. faecalis) were used as assessment methods. The results showed a 99.97 % reduction on CFU counts of S. aureus and 99.999998 % reduction on E. faecalis (using both light sources). It is concluded that AmPDT carried out with 1,9-dimethylmethylene blue associated with either Laser, LED or Polarized light was efficacious on the reduction, in vitro, of the counts of aerobes and aerotolerant anaerobes Gram-positive bacteria.
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Antonio L. B. Pinheiro, Fernando J. P. Sampaio, Darcy de A. Santos, Pedro Jorge L. Crugeira, Susana C. P. S. de Oliveira, Juliana S. C. Monteiro, Sandra R. C. A. Fagnani, Iago P. F. Nunes, Luiz G. P. Soares, and Paulo Fernando de Almeida "Nanoconcentrations of 1,9-dimethylmethylene blue (DMMB) associated to laser, LED or polarized light are highly effective on AmPDT carried out in aerobes and aerotolerant anaerobes Gram-positive bacteria", Proc. SPIE 11221, Mechanisms of Photobiomodulation Therapy XV, 112210P (11 March 2020); https://doi.org/10.1117/12.2543732
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