Fast volumetric mapping of human brain slices

Luca Pesce; Annunziatina Laurino; Vladislav Gavryusev; Giacomo Mazzamuto; Giuseppe Sancataldo; Marina Scardigli; Matteo Roffilli; Ludovico Silvestri; Irene Costantini; Francesco Saverio Pavone

doi:10.1117/12.2544133

21 February 2020 Fast volumetric mapping of human brain slices

Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, Francesco Saverio Pavone

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Proceedings Volume 11226, Neural Imaging and Sensing 2020; 112260B (2020) https://doi.org/10.1117/12.2544133
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We still lack a detailed map of the anatomical disposition of neurons in the human brain. A complete map would be an important step for deeply understanding the brain function, providing anatomical information useful to decipher the neuronal pattern in healthy and diseased conditions. Here, we present several important advances towards this goal, obtained by combining a new clearing method, advanced Light Sheet Microscopy and automated machine-learning based image analysis. We perform volumetric imaging of large sequentially stained human brain slices, labelled for two different neuronal markers NeuN and GAD67, discriminating the inhibitory population and reconstructing the brain connectivity.

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Luca Pesce, Annunziatina Laurino, Vladislav Gavryusev, Giacomo Mazzamuto, Giuseppe Sancataldo, Marina Scardigli, Matteo Roffilli, Ludovico Silvestri, Irene Costantini, and Francesco Saverio Pavone "Fast volumetric mapping of human brain slices", Proc. SPIE 11226, Neural Imaging and Sensing 2020, 112260B (21 February 2020); https://doi.org/10.1117/12.2544133

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