In vivo mouse brain imaging through the thinned skull with 1700 nm optical coherence microscopy (Conference Presentation)

Jun Zhu; Vivek J. Srinivasan

doi:10.1117/12.2549135

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9 March 2020 In vivo mouse brain imaging through the thinned skull with 1700 nm optical coherence microscopy (Conference Presentation)

Jun Zhu, Vivek J. Srinivasan

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Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 112280P (2020) https://doi.org/10.1117/12.2549135
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Central nervous system diseases start at the microscopic level; thus, in vivo deep brain imaging with cellular resolution is needed. Using the 1700 nm optical window, which has lowest ballistic attenuation for brain imaging, an optical coherence microscopy system was designed for in vivo imaging of mouse brain cellular architecture. Taking advantage of relatively low scattering at 1700 nm, neuronal cell bodies in the mouse brain were visualized through a thinned skull preparation, which minimizes inflammation and preserves intracranial pressure. Cellular architecture was co-registered with simultaneous angiographic imaging, showing the distribution of neuronal cell bodies relative to supplying capillaries.

Conference Presentation

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Jun Zhu and Vivek J. Srinivasan "In vivo mouse brain imaging through the thinned skull with 1700 nm optical coherence microscopy (Conference Presentation)", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112280P (9 March 2020); https://doi.org/10.1117/12.2549135

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