Quantification of ex vivo tissue activity by short and long time-course analysis of multifunctional OCT signals

I. G. Abd El-Sadek; A. Miyazawa; L. T. W. Shen; S. Fukuda; T. Yamashita; Y. Oka; P. Mukherjee; S. Makita; S. Matsusaka; T. Oshika; H. Kano; Y. Yasuno

doi:10.1117/12.2548541

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21 February 2020 Quantification of ex vivo tissue activity by short and long time-course analysis of multifunctional OCT signals

I. G. Abd El-Sadek, A. Miyazawa, L. T. W. Shen, S. Fukuda, T. Yamashita, Y. Oka, P. Mukherjee, S. Makita, S. Matsusaka, T. Oshika, H. Kano, Y. Yasuno

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Proceedings Volume 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV; 112282C (2020) https://doi.org/10.1117/12.2548541
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

We present a new OCT-based tissue dynamics/subcellular motion analysis method to visualize tissue dynamics, where we increase the functionality of OCT to be sensitive for tissue dynamics by utilizing rapid-time-sequence analysis of OCT signals. These analysis includes log intensity variance (LIV) and OCT time-correlation analysis (OCT decorrelation speed; OCDS). In addition to LIV and OCDS methods, attenuation coefficient (AC), birefringence, and degree of polarization uniformity (DOPU) analysis were performed. These methods used to visualize and quantify long-term tissue dynamics degradation of different tissue types such as dissected mouse liver and tumor spheroids. These methods were quantitative, so the time-course tissue dynamics degradation has been not only visualized as an image, but also quantitative analysis of the dynamics degradation were performed.

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I. G. Abd El-Sadek, A. Miyazawa, L. T. W. Shen, S. Fukuda, T. Yamashita, Y. Oka, P. Mukherjee, S. Makita, S. Matsusaka, T. Oshika, H. Kano, and Y. Yasuno "Quantification of ex vivo tissue activity by short and long time-course analysis of multifunctional OCT signals", Proc. SPIE 11228, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXIV, 112282C (21 February 2020); https://doi.org/10.1117/12.2548541

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