Single cell radiometry using droplet optofluidics

Byunghang Ha; Tae Jin Kim; Ejung Moon; Guillem Pratx

doi:10.1117/12.2542463

21 February 2020 Single cell radiometry using droplet optofluidics

Byunghang Ha, Tae Jin Kim, Ejung Moon, Guillem Pratx

Proceedings Volume 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII; 112350S (2020) https://doi.org/10.1117/12.2542463
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Single cell analysis has become a crucial goal since cellular heterogeneity was acknowledged as one of the greatest challenges in cancer therapeutics. Radioactive probes can play an important role in single cell study thanks to its unique capability of tracking small molecules with minimal modification of their chemical structure. Our lab has recently developed a new technique known as radioluminescence microscopy (RLM) to measure the amount of radiotracer in single cells. Yet, RLM can image only up to about 100 cells and lacks capability of integrating with subsequent sample processing such as cell sorting. Here we introduce a robust, high throughput single cell radiometry based on radiofluorogenesis and droplet optofluidics. As an example, we quantitated [¹⁸F]-fluorodeoxyglucose radiotracer uptake in single human breast cancer cells and assessed cellular heterogeneity in single cell metabolism.

Citation Download Citation

Byunghang Ha, Tae Jin Kim, Ejung Moon, and Guillem Pratx "Single cell radiometry using droplet optofluidics", Proc. SPIE 11235, Microfluidics, BioMEMS, and Medical Microsystems XVIII, 112350S (21 February 2020); https://doi.org/10.1117/12.2542463

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
4 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Radiometry

Cancer

Microfluidics

Optofluidics

Positron emission tomography

Breast cancer

Show All Keywords

Keywords/Phrases

Search In:

Publication Years