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31 March 2020 Optimization of curcumin formulations for fluorescence-based applications
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Curcumin is a natural compound used for medical and food-industrial applications due to its antioxidant, antiinflammatory, antifungal, antiviral, and antiseptic properties. In addition to these applications, curcumin can be used for fluorescence labeling in disease diagnostics/theranostics and monitoring photodynamic antimicrobial inactivation. In terms of fluorescence-based applications, the sensitivity to detect curcumin depends on the curcumin bioavailability or the formulation concentration. In this study, we used 375 nm and 445 nm laser excitation to characterize the wavelength dependent fluorescence spectra for curcumin in ethanol solutions of 10, 20, 50 and 75 μg/ml (i.e., 27.15, 54.30, 135.75 and 203.625 μM, respectively). Our results suggested the fluorescence intensity as a function of concentration saturates around 135.75 μM. Fluorescence intensity increase as a function of concentration was observed between 650 nm and 800 nm for the 445 nm excitation. Increase in the same wavelength range was obtained for the difference between the fluorescence spectra generated between the 375 nm and 445 nm excitation wavelengths. The spectral features reported in our study can be used on the design of curcumin formulations using Sigma Aldrich curcumin C7727 for fluorescencebased applications, determination of bioavailability of curcumin formulations and photodynamic dosimetry.
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Marcelo Saito Nogueira, Fabio Francisco Pinto Jr., Raphael Antonio Caface, Kleber Thiago de Oliveira, Vanderlei Salvador Bagnato, and Francisco Eduardo Gontijo Guimarães "Optimization of curcumin formulations for fluorescence-based applications", Proc. SPIE 11238, Optical Interactions with Tissue and Cells XXXI, 112381F (31 March 2020);

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