Confocal Raman microspectroscopy for evaluation of optical clearing efficiency of the skin ex vivo

I. Yu. Yanina; J. Schleusener; J. Lademann; V. V. Tuchin; M. E. Darvin

doi:10.1117/12.2550352

21 February 2020 Confocal Raman microspectroscopy for evaluation of optical clearing efficiency of the skin ex vivo

I. Yu. Yanina, J. Schleusener, J. Lademann, V. V. Tuchin, M. E. Darvin

Proceedings Volume 11239, Dynamics and Fluctuations in Biomedical Photonics XVII; 112390W (2020) https://doi.org/10.1117/12.2550352
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Optical clearing (OC) of biological tissues is a promising technology for a wide spread use in medical practice to increase the screening depth, spatial resolution and contrast of the resulting images/spectra. Nevertheless, despite the significant OC effect, some biocompatible optical clearing agents (OCAs) can adversely affect biological tissues, causing local hemostasis, morphological changes, d ehydration, and in some cases even tissue necrosis. The aim of this study was to study the effect of Omnipaque 300 and fructose solutions of various concentrations and exposure times on the intact skin using confocal Raman microspectroscopy. It was shown that the application of each of these OCAs on intact skin for 5 min also leads to an appreciable OC effect. The increase in OC was achieved using a mixture of Omnipaque 300 with DMSO; it was shown that the optical properties of the skin can be controlled at a depth of about 80 μm.

Citation Download Citation

I. Yu. Yanina, J. Schleusener, J. Lademann, V. V. Tuchin, and M. E. Darvin "Confocal Raman microspectroscopy for evaluation of optical clearing efficiency of the skin ex vivo", Proc. SPIE 11239, Dynamics and Fluctuations in Biomedical Photonics XVII, 112390W (21 February 2020); https://doi.org/10.1117/12.2550352

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KEYWORDS

Skin

Raman spectroscopy

Confocal microscopy

Optical clearing

Imaging spectroscopy

Tissues

Water

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