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6 March 2020 In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)
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Abstract
Surgical removal of the head cuticle of Drosophila is necessary for optical brain imaging, leading to the Drosophila death in a short period of time and thus hindering long term monitoring. Targeting to the unmet need of surgery free procedure for Drosophila brain researches, in this study, laser scanning optical resolution photoacoustic microscopy (LSOR-PAM) of in vivo cuticle intact RCaMP labeled Drosophila mushroom body (MB) is presented. The MB is a higher-order olfactory center in Drosophila brain. This study paves the way toward exploring LSOR-PAM functional imaging capability of cuticle intact Drosophila brain under olfactory stimulus.
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Kai-Yao Chang, Wen-Hung Shih, Shun-Chi Wu, Kwok Ho Lam, Yen-Yin Lin, Ann-Shyn Chiang, and Meng-Lin Li "In vivo cuticle intact Drosophila mushroom body imaging using laser scanning optical resolution photoacoustic microscopy (Conference Presentation)", Proc. SPIE 11240, Photons Plus Ultrasound: Imaging and Sensing 2020, 112401Z (6 March 2020); https://doi.org/10.1117/12.2547187
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