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9 March 2020 A simple, compact and robust phase and fluorescence microscope for cell cycle study (Conference Presentation)
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Abstract
We designed a simple, compact and robust microscope for phase and fluorescent imaging. No mechanical movement of neither sample nor objective or any other part of the system is needed to change between the phase-contrast and fluorescence modality. We can observe a thousand cells in parallel in a single field of view with resolution down to 2 µm. We demonstrate the system on a FUCCI marked HeLa cell culture observed over several days directly in the standard incubator. We compare the obtained statistics to the flow cytometer data and show that we can produce a statistically relevant time-resolved measurement
Conference Presentation
© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ondrej Mandula, Cedric Allier, Dainel Fiole, Jean-Philippe Kleman, Francoise Lacroix, Lionel Hervé, and Sophie Morales "A simple, compact and robust phase and fluorescence microscope for cell cycle study (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430P (9 March 2020); https://doi.org/10.1117/12.2543764
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