Dynamic signatures of lipid droplets reveal cellular metabolic changes associated with stimuli and drug treatments (Conference Presentation)

Chi Zhang; Stephen A. Boppart

doi:10.1117/12.2545387

9 March 2020 Dynamic signatures of lipid droplets reveal cellular metabolic changes associated with stimuli and drug treatments (Conference Presentation)

Chi Zhang, Stephen A. Boppart

Author Affiliations +

Proceedings Volume 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII; 112430V (2020) https://doi.org/10.1117/12.2545387
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Coherent anti-Stokes Raman scattering (CARS) microscopy was used to disclose dynamic information from lipid droplets (LDs) in living cancer cells. Statistical analyses of LD dynamics show different pools of LDs associated with LD synthesis and degradation processes. We found that each LD population satisfies a log-normal distribution, thus allowing quantitative comparison through fitting of population histograms. Two-dimensional analyses of LD displacements and speeds offer dynamic signatures of cells to monitor responses to stimuli and drugs. We revealed hypothermia-induced cellular metabolic changes and a two-step metabolic response during apoptosis. We also explored dynamic signatures of LDs during starvation and various drug exposures.

Conference Presentation

Citation Download Citation

Chi Zhang and Stephen A. Boppart "Dynamic signatures of lipid droplets reveal cellular metabolic changes associated with stimuli and drug treatments (Conference Presentation)", Proc. SPIE 11243, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XVIII, 112430V (9 March 2020); https://doi.org/10.1117/12.2545387

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available