Two-photon phosphorescence lifetime microscopy (2PLM) of Oxygen (Conference Presentation)

Sergei A. Vinogradov

doi:10.1117/12.2550690

9 March 2020 Two-photon phosphorescence lifetime microscopy (2PLM) of Oxygen (Conference Presentation)

Sergei A. Vinogradov

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 1124416 (2020) https://doi.org/10.1117/12.2550690
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Molecular oxygen plays a unique role in cellular energy metabolism by serving as the terminal electron acceptor in the mitochondrial respiratory chain. Quantitative imaging of oxygen can provide invaluable information about metabolism in normal and diseased states. Two-photon phosphorescence lifetime microscopy (2PLM) enables imaging of oxygen concentration gradients in 3D with micron-scale resolution. The method has found use in neuroscience, stem cell and tumor biology. We will discuss the principles of 2PLM and focus on the new phosphorescent probes, which enable imaging of oxygen simultaneously with imaging of temperature.

Conference Presentation

Citation Download Citation

Sergei A. Vinogradov "Two-photon phosphorescence lifetime microscopy (2PLM) of Oxygen (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 1124416 (9 March 2020); https://doi.org/10.1117/12.2550690

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