Real time imaging of the detection volume of a confocal microscope

Barun Kumar Maity; Sudipta Maiti

doi:10.1117/12.2544129

14 February 2020 Real time imaging of the detection volume of a confocal microscope

Barun Kumar Maity, Sudipta Maiti

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441D (2020) https://doi.org/10.1117/12.2544129
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

Deconvolution, pixel reassignment or adaptive optics-based strategies utilize information about the detection profile in improving the resolution of optical microscopy. Here, we show a novel method which allows us to obtain the single-photon detection volume of a laser scanning confocal microscope at any desired location of the object. It can create a stationary, virtual ‘guide star’ at the chosen location while the excitation beam is scanning the sample, by using an optical fiber placed in the non-descanned path of the microscope. Our experimental results are verified by diffraction theory-based calculations. The major advantages of our method are that it is alignment free, affordable, sensitive and applicable to many different modes of confocal imaging.

Conference Presentation

Citation Download Citation

Barun Kumar Maity and Sudipta Maiti "Real time imaging of the detection volume of a confocal microscope", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441D (14 February 2020); https://doi.org/10.1117/12.2544129

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