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9 March 2020 Multiphoton FLIM is gaining ground as a clinical tool (Conference Presentation)
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Abstract
Multiphoton fluorescence lifetime imaging (FLIM) is gaining ground as a non-invasive and very sensitive method in life sciences, and even as a clinical tool. First clinical devices employing FLIM are on the market, e.g. MPTflex. A hot topic is using metabolic imaging to investigate melanoma lesions (Fig.1). This method utilizes imaging of the ratio of the amounts of the free and protein-bound forms of the intracellular autofluorescent metabolic co-enzyme nicotinamide adenine dinucleotide (NADH) [1,2,3,4]. In another study, we investigated safety aspects of nanoparticle based sun screens. Multiphoton FLIM enables tracing of nanoparticles after application on the skin [5]. Furthermore, in case of penetration into the viable epidermis metabolic imaging can be employed to investigate toxicity on skin cells [6].
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© (2020) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hauke Studier, Yousuf S. Mohammed, Michael S. Roberts, Amy Holmes, Michael Pastore, and Wolfgang Becker "Multiphoton FLIM is gaining ground as a clinical tool (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441Q (9 March 2020); https://doi.org/10.1117/12.2544795
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