Rapid volumetric multiphoton microscopy via dual resonant scanning with deep learning (Conference Presentation)

Chia Wei Hsu; Yu-Shin Hsu; Chun-Yu Lin; Shean-Jen Chen

doi:10.1117/12.2546860

9 March 2020 Rapid volumetric multiphoton microscopy via dual resonant scanning with deep learning (Conference Presentation)

Chia Wei Hsu, Yu-Shin Hsu, Chun-Yu Lin, Shean-Jen Chen

Author Affiliations +

Proceedings Volume 11244, Multiphoton Microscopy in the Biomedical Sciences XX; 112441W (2020) https://doi.org/10.1117/12.2546860
Event: SPIE BiOS, 2020, San Francisco, California, United States

Abstract

A tunable acoustic gradient lens and a resonant mirror are integrated into a multiphoton excited microscope that is rapidly and precisely controlled by an embedded field programmable gate array to acquire images at 8 kHz for x-z plane. The pulse train signal of a femtosecond laser provides up to 95 MHz voxel rate, and one pulse per pixel. A volumetric image rate at up to 30 Hz can be realized according to the precise focal position and the excited signal, and deep learning. Biological images can be demonstrated at high-speed volumetric imaging rate.

Conference Presentation

Citation Download Citation

Chia Wei Hsu, Yu-Shin Hsu, Chun-Yu Lin, and Shean-Jen Chen "Rapid volumetric multiphoton microscopy via dual resonant scanning with deep learning (Conference Presentation)", Proc. SPIE 11244, Multiphoton Microscopy in the Biomedical Sciences XX, 112441W (9 March 2020); https://doi.org/10.1117/12.2546860

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